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Immunogold-TEM characterization of HT-22 exosomes. (A) IgG control for single immunogold labeling. (B) Single immunogold labeling shows that exosome-like vesicles secreted by HT-22 cells express Wnt3a, Wnt5a, Wnt7a, and GPR177. (C) IgG control for double immunogold. (D) Doubled immunogold analysis detected co-expression of Wnt5a and GPR177. Red and black arrows show the presence of Wnt5 and GPR177, respectively.
