Figure 2.

Disruption of bacterial biofilms by endolysins. (A) Antibiofilm activity of endolysins against Klebsiella pneumoniae Ts 104-14, A. baumannii Ts 50-16, and Pseudomonas aeruginosa Ts 38-16 biofilms stained with crystal violet. OD₆₀₀ was measured after 2h of exposure to endolysins. Dash line indicates the cutoff value calculated as three SDs above the mean OD of the negative control. Data are shown as mean±SD. Asterisk (^*) indicates significant difference in optical density compared to control without the enzyme treatment of biofilm (p<0.05, one-way ANOVA). (B) Light and scanning electron microscopy images of lysis of A. baumannii Ts 50-16 preformed biofilms on glass slides after a 2-h exposure to endolysins in 100mg/ml concentration.