Figure 3.

The neutralization effects of immunized serum (A) and specific antibodies (B) toward four endolysins. LysAm24, LysECD7, LysAp22, and LysSi3 were preincubated with endolysin-immunized rabbit serum, nonimmunized serum, PBS solution, or affinity-purified antibodies for 10min at 37°C. Each mixture was then added to the growing culture of A. baumannii Ts 50-16, and CFU count was estimated after 30-min incubation. Data are shown as mean±SD (one-way ANOVA). Asterisk (^*) indicates significant difference in activity compared to nonimmunized serum (A) or control in PBS (B; p < 0.05).