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. 2021 Oct 18;22(6):839. doi: 10.3892/ol.2021.13100

Figure 2.

Figure 2.

Identification of the regulatory effect of circRNA-FOXO3 on miR-543 in non-small cell lung cancer cells. (A) Top 50 upregulated and downregulated miRNAs between the mock and circRNA-FOXO3 groups are illustrated in a heatmap. Green represents downregulated, while red represents upregulated. (B) Venn diagram showing the mutual putative target miRNAs of circRNA-FOXO3 and Foxo3 mRNA. (C) Top 6 altered (log10 FC) miRNAs screened using a miRNA microarray and online prediction. (D) Putative miR-543 binding sites with respect to circRNA-FOXO3. (E) Reverse transcription-quantitative PCR was used to detect miR-543 expression in A549 cells transfected with miR-543 mimics or miR-NC. (F) Luciferase activity of A549 cells co-transfected with circRNA-FOXO3 vector and indicated miRNA mimics. (G) RNA pull-down assay indicating circRNA-FOXO3 binding to miR-543. (H) Relative expression levels of circRNA-FOXO3 in A549 cells transfected with circRNA vectors or miR-543 mimics. *P<0.05. circRNA, circular RNA; FC, fold-change; miR/miRNA, microRNA; NC, negative control.