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. 2021 Oct 18;22(6):839. doi: 10.3892/ol.2021.13100

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Foxo3 is a target of miR-543 in NSCLC cells. (A) Online bioinformatics prediction tools revealed the binding sequences of miR-543 and the Foxo3 mRNA 3′-untranslated region. (B) Relative expression levels of Foxo3 in NSCLC tissues and non-tumor tissues. (C) Spearman's correlation analysis was used to reveal the negative correlation between Foxo3 and miR-543 expression in NSCLC tissues. (D) Luciferase activity of the Foxo3 vector in A549 cells co-transfected with miR-543 mimics or miR-NC. (E and F) Western blot analysis revealed the relative protein expression levels of Foxo3 in A549 cells transfected with anti-miR-543, anti-miR-NC, miR-543 mimics or miR-NC. *P<0.05. miR/miRNA, microRNA; NC, negative control; NSCLC, non-small cell lung cancer.