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. 2021 Oct 18;22(6):839. doi: 10.3892/ol.2021.13100

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Foxo3 overexpression reverses the effects of miR-543 silencing on glycolysis and cisplatin resistance. (A) Western blot analysis revealed the relative protein expression levels of Foxo3 in A549 and H1975/DDP cells transfected with Foxo3 or negative control vector. (B) Relative expression levels of miR-543 in A549 cells transfected with anti-miR-543 or anti-miR-NC. (C) Glucose consumption was determined in A549 cells transfected with Foxo3 vectors and/or anti-miR-543. (D) Lactate production of A549 cells transfected with Foxo3 vectors and/or anti-miR-543. (E) A CCK-8 assay was performed to determine cell proliferation following DDP treatment of A549 cells transfected with Foxo3 vectors and/or anti-miR-543. (F) Relative glucose consumption and (G) lactate production of H1975/DDP cells transfected with Foxo3 vectors and/or anti-miR-543. (H) A CCK-8 assay was performed to determine cell proliferation following DDP treatment of H1975/DDP cells transfected with Foxo3 vectors and/or anti-miR-543. *P<0.05. CCK-8, Cell Counting Kit-8; DDP, cisplatin; miR/miRNA, microRNA; NC, negative control.