Figure 4.

Influence of palbociclib on cell cycle of different cell lines. (A) Palbociclib arrest cells in the G1 phase of cell cycle by blocking the S/G1 phase checkpoint. (B) Representative histograms depicting the gating strategy of Hoechst staining (DNA) in breast cancer cell line MCF-7 after treatment (Co/2 µM/2 Gy/2 µM + 2 Gy). (C) Population of cells in the G0/G1 or G2/M phase, respectively. Cells were treated with 2 µM palbociclib, 2 Gy dose irradiation, or a combination of both. Each value represents mean ± SD (n = 4). Significance was determined by one-tailed Mann–Whitney U test *p ≤ 0.05, **p = 0.01.