Video 5.
Six-color imaging of cells CUP1p-expressing Whi5-mTFP1 (OAM455) during quiescence entry. Notice that high-Cdk1 Q-cells expressing CUP1p-WHI5-mTFP1 keep Whi5-mTFP1 in the cytoplasm during starvation (budded cell in the middle). Cells also carry the cell cycle marker Cdc10-mCyOFP1 and the stress response markers Sfp1–mScarlet-I, Gln3-mKOκ, Rtg1-mNeptune2.5, and Xbp1-mNeonGreen. Each peripheral hexagon displays a median-filtered fluorescent channel. Sampling rate = 12 min. Hexagon side = 40 µM. Playback = 60 frames per second.