Video 7.
Six-color imaging of 6C1 cells (OAM425) during cyclic exposure to stress episodes triggered by hourly changes between rich (SCD) and glucose-deficient (synthetic complete glycerol) medium. Cells carry Cdc10-mCyOFP1 as cell cycle marker and Sfp1–mScarlet-I, Gln3-mKOκ, Rtg1-mNeptune2.5, Stb3-mTFP1, and Xbp1-mNeonGreen as stress response markers. Each peripheral hexagon displays a median-filtered fluorescent channel. Sampling rate = 12 min. Hexagon side = 45 µM. Playback = 60 frames per second.