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. 2021 Oct 14;22(6):1450. doi: 10.3892/etm.2021.10885

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Copyright: © Ibrahim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Agarose gel electrophoresis of multiplex-PCR amplification products. (A) Acinetobacter baumannii (lanes 1 and 3) and Klebsiella pneumoniae (lane 5) showed positive expression of the bla_TEM gene (800 bp). Acinetobacter baumannii (lanes 2, 4 and 6) and Escherichia coli (lane 7) showed negative findings to ESBL gene expression. (B) Klebsiella pneumoniae (lane 1), Escherichia coli (lanes 2, 3 and 6) and Enterobacter cloacae (lane 4), positive bla_CTX-M gene (688 bp). Acinetobacter baumannii (lane 5) and Proteus mirabilis (lane 7) showed negative findings to ESBL gene expression. (C) Lane 2, co-existence of bla_TEM and bla_SHV genes in Klebsiella pneumoniae isolate. Acinetobacter baumannii (lanes 3 and 6) showed positive bla_TEM gene expression. Klebsiella pneumoniae (lane 4) showed positive bla_SHV gene expression (713 bp). Acinetobacter baumannii (lanes 1, 5 and 7) showed negative findings to ESBL gene expression. Lane M, DNA marker (1,500-100 bp);. ESBL, extended-spectrum β-lactamase.