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. 2021 Oct 12;22(6):1439. doi: 10.3892/etm.2021.10874

Figure 4.

Figure 4

Hypoxia regulates the expression of RP11-531A24.3, ANXA2 and HIF-1α in HA-VSMCs. Effect of hypoxia on the expression of (A) RP11-531A24.3 *P<0.05 vs. 24 h; #P<0.05 vs. 12 h; &P<0.05 vs. 6 h and (B) ANXA2, aP<0.05 vs. 24 h; bP<0.05 vs. 12 h; cP<0.05 vs. 6 h; and HIF-1α, dP<0.05 vs. 24 h; eP<0.05 vs. 12 h; fP<0.05 vs. 6 h. (C) HIF-1α expression at protein levels under hypoxic conditions was detected in HA-VSMCs transfected with LV-OE or LV-mock. **P<0.01 vs. LV-mock. (D) HIF-1α expression at the protein level was detected in HA-VSMCs transfected with si-NC, si-ANXA2 and blank control. *P<0.05 vs. si-NC; #P<0.05 vs. Blank. (E) HIF-1α and ANXA2 expression levels were detected in HA-VSMCs transfected with si-NC, si-HIF-1α and blank control. **P<0.01 vs. si-NC. Data are presented as the mean ± SD (n=3). HA-VSMCs, human aorta-vascular smooth muscle cells; ANXA2, annexin A2; HIF-1α, hypoxia inducible factor-1α; LV-mock, empty lentiviral vector; LV-OE, lentiviral RP11-531A24.3 overexpression vector; si, small interfering; NC, negative control.