FIGURE 7.

Enhanced ex vivo bT_RM recall responses following α-PD-1 Ab treatment. BMNCs were obtained from BALB/c animals at 30 days post prime-CNS boost and were stimulated ex vivo with AI9 peptide. The cells were then left untreated or treated with α-PD-1 for 4–6 hr prior to intracellular staining. (a) Representative contour plots present expression of IFN-γ and Ki67 by CD103⁺CD8⁺ T-cells. (b,c) Bar graphs of pooled data show frequencies of IFN-γ expression, as well as Ki67 production, by CD103⁺CD8⁺ T-cells. Data shown are representative of three experiments (n = 3 animals/group). **p ≤ .01