Fig. 3.

ZIP10 increases ITGA10 expression in osteosarcoma. a Gene array analysis of NC and shZIP10 Saos-2 cells. Up−/down-regulated genes associated with the integrin family are shown. b qRT-PCR analysis of integrins in Saos-2. c Expression levels of AKT, p-AKT, PAK, p-PAK, p-FAK and p-SRC were analyzed by WB using 143B and Saos-2 cells with/without ITGA10 knockdown. d WB analysis of AKT and PAK activation with/without ZIP10 overexpression in 143B cells. e Protein levels of p-AKT and p-PAK were quantified using ImageJ software. f A CCK-8 assay was performed to determine cell proliferation. g Flow cytometry was conducted to determine apoptosis. Apoptosis rates were quantified. h The whole-cell extract of 143B cells treated with/without cisplatin (Cis) for 72 h was subjected to WB analysis. Data are means ± SEM; *P < 0.05, **P < 0.01