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. 2021 Aug 24;7(8):000644. doi: 10.1099/mgen.0.000644

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© 2021 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 5. — blast comparisons of IncB/O/K/Z plasmids for three MSM S. sonnei isolates in England, obtained with Nanopore sequencing. These are compared to a plasmid known to be associated with MSM in Australia, pAUSMDU00008333 plasmid 3 (LR213460.1). Three S. sonnei plasmids are from this study (isolates 598080; pink ring, 607387; green ring and 893916; blue ring) and the outer peach coloured ring is pAUSMDU00008333 plasmid 3. Genes present are shown in red on the outer ring (excluding hypothetical proteins). aph(3′)-Ib (strA): Aminoglycoside 3′-phosphotransferase; aph(6)-Id (strB): Aminoglycoside O-phosphotransferase; sul2: Dihydropteroate synthase; tet(A): Tetraycline efflux protein; psiB: Plasmid SOS inhibition protein B; noc: Nucleoid occlusion protein; ssb: Single-stranded DNA-binding protein; Anti-Restriction protein; yhdJ: DNA adenine methyltransferase; umuD and umuC - UV mutagenesis and repair proteins; cysE: Serine acetyltransferase; hin: DNA-invertase; aer: Aerotaxis receptor; rfaH: Transcription antitermination protein; yqiJ and yqiK: Inner membrane proteins; bfpB: Outer membrane lipoprotein. Figure produced with blast Ring Image Generator (BRIG) [52] and gradient of blast identity is shown in the legend.