Extended Data Fig. 6. Iboxamycin (IBX) efficiently arrests translation at the start codon.

Toeprinting analysis showing sites of IBX-induced translation arrest of ErmBL and ErmDL leader peptides. Because all reactions contained mupirocin, an inhibitor of Ile-tRNA synthetase, the ribosomes that escape inhibition by ribosome-targeting antibiotics are trapped at the codon preceding Ile (black arrowheads). Red arrowheads mark translation arrest at the start codon, while cyan arrowheads denote known erythromycin-induced arrest sites D10 (ermBL) and L7 (ermDL). Each gel is representative of two independent experiments, for source data see Supplementary Figure 1. ERY, erythromycin; CLI, clindamycin.