Skip to main content
. 2021 Oct 24;30:09636897211052959. doi: 10.1177/09636897211052959

Figure 4.

Figure 4.

circRFWD2 could talk with miR-6817-5p through BMPR2. (A) The putative binding sites between miR-6817-5p and circRFWD2 was displayed. (B) 40 ng circRFWD2 luciferase reporter plasmids with 100 nM miR-NC or miR-6817-5p mimic were co-transfected into HEK 293 T cells, luciferase assays were conducted 48 h after transfection. (C) The putative binding sites of miR-6817-5p to the 3’ untranslated region of BMPR2 predicted by TargetScanhuman 7.2 database, and the mutation site in the 3’ UTR of BMPR2 was shown. (D) 40 ng BMPR2 luciferase reporter plasmids with 100 nM miR-NC or miR-6817-5p mimic were co-transfected into HEK 293 T cells, luciferase assays were conducted 48 h after transfection. (E) Western blot analysis of BMPR2 in miR-NC, miR-6817-5p mimic, and miR-6817-5p inhibitor, si-NC, si-circRFWD2, si-circRFWD2/miR-6817-5p inhibitor, and si-circRFWD2/miR-6817-5p mimic groups. (F) The mRNA levels of BMPR2 in miR-NC, miR-6817-5p mimic, and miR-6817-5p inhibitor, si-NC, si-circRFWD2, si-circRFWD2/miR-6817-5p inhibitor, and si-circRFWD2/miR-6817-5p mimic groups. (G) The protein levels of BMPR2 in miR-NC, miR-6817-5p mimic, and miR-6817-5p inhibitor, si-NC, si-circRFWD2, si-circRFWD2/miR-6817-5p inhibitor, and si-circRFWD2/miR-6817-5p mimic groups. GAPDH is a protein-loading control. All experiments were repeated at least three times. The data are shown as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. si-circRFWD2: small interfering RNAs targeting circRFWD2; si-NC: small interfering RNA negative control; NC: negative control.