FIG 3.

S. enterica, but not E. coli, enhances CVB3 stability. CVB3 decay at 37°C when incubated with DMEM (slope, −0.06651; R², 0.6940) of 10⁸ of S. enterica (slope, 0.001960; R², 0.9337) (A) or 10⁸ of E. coli (slope, −0.06700; R², 0.8773) (B). The first-order decay rate for CVB3 was determined by a best-fit line generated by fitted linear regression as previously described (47). (C) Rates of CVB3 decay in either PBS, 10⁸ CFU of S. enterica, or 10⁸ CFU of E. coli. ***, P < 0.001; ****, P < 0.0001; one-way ANOVA. (D) Rates of CVB3 decay in either PBS or 10⁸, 10⁶, 10⁴, or 10² CFU of S. enterica. (E) Rates of CVB3 decay in either PBS or 10⁸, 10⁶, 10⁴, or 10² CFU of E. coli. For all, the data represent 4 to 6 independent experiments (n = 8 to 12); bars represent mean ± SEM; **, P < 0.01; one-way ANOVA.