FIG 2.

Effect of nectin-1 siRNA knockdown on VZV infection of human neurons. (A) RNA fold changes normalized to HRPT1 expression. The error bars indicate the SEM (P values refer to two-way analysis of variance with Bonferroni posttest). (B) Effect of siRNA-mediated knockdown against GAPDH or nectin-1 (50 μM) at 7 and 10 days by Western blotting with whole-cell lysates. (C) Effect of siRNA-mediated knockdown on cell surface level of nectin-1 by flow cytometry at 7 days posttransfection. (D) Neurons transfected with siRNA against nectin-1 (7 days) were infected with VZV, and the effect of nectin-1 knockdown on VZV infection was analyzed by Western blotting for VZV gE expression at 4 days after infection. (E) Dose-dependent effect of nectin-1 siRNA (0, 10, 25, and 50 μM) on VZV infection was analyzed by Western blotting with anti-VZV gE and pORF63 antibodies.