FIG 3.

Soluble nectin-1 influences VZV infectivity of neurons. (A) Neurons were preincubated with soluble VZV gE (sgE) and infected with VZV for 4 days. Dose-dependent effect of sgE treatment on VZV infection was analyzed by Western blotting with anti-VZV gE antibody. BSA was used as an experimental control. (B) Neurons preincubated without (lane 2) or with 100 μg of nectin-1(Bac) produced in Sf-9 cells (lane 3) were infected with VZV, and whole-cell lysate collected 4 days after infection was subjected to Western blotting with anti-VZV gE antibody. (C) Effect of preincubation of soluble nectin-1 (sNectin-1) produced in NS0 cells with HSV-1 was analyzed by Western blotting with anti-HSV gD antibody. (D) VZV was preincubated with increasing doses of sNectin-1 prior to infection of neurons. Four days later, whole-cell lysates were collected and subjected to Western blotting with anti-VZV gE antibody. Anti-GAPDH antibody was used as an internal control. (E) VZV was preincubated with 20 μg of Nectin-1(FL) produced in HEK293T cells prior to infection of neurons. Four days later, whole-cell lysates were collected and subjected to Western blotting using anti-VZV gE antibody. Anti-GAPDH antibody was used as internal control.