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Addition of soluble nectin-1 during, but not after, infection inhibits VZV infectivity of neurons. (A) Neurons were infected with VZV in the presence of increasing concentrations of sNectin-1 during infection (left) or at 2 h postinfection (right). Whole-cell lysates were collected at 4 days after infection and subjected to Western blotting with anti-VZV gE and pORF63 antibody. Anti-GAPDH antibody was used as an internal control. (B) BSA was used as an experimental control.
