FIG 2.

Impact of TAX1BP1 depletion on RSV replication in cells. A549 cells were transfected with control siRNA (siCT) or siRNA targeting TAX1BP1 (siTAX1BP1) and then infected 24 h later with either rHRSV-mCherry or rHRSV-Luc at an MOI of 0.5. (A) RSV replication was quantified at 48 h postinfection by measurement of fluorescence (left) and luminescence (right), expressed in arbitrary units (A.U.), in cell lysates. Data are representative of results from three experiments made in quadruplicates. Data are means ± standard errors of the means (SEM). *, P < 0.05. (B) Western blot analysis of TAX1BP1 silencing and RSV-N expression in cells infected with either rHRSV-mCherry or rHRSV-Luc, at 48 h postinfection. (C) Titration of virions released into the culture media of cells treated with siCT (left) and siTAX1BP1 (right) and infected with rHRSV-mCherry (top) or rHRSV-Luc (bottom). Calculated viral titers in PFU per milliliter are indicated.