FIG 7.

Kinetics of fluorescent expression in the lungs of K18 hACE2 transgenic mice treated with 1212C2 or 1213H7 hMAbs and infected with rSARS-CoV-2 Venus and rSARS-CoV-2 mCherry SA. Six- to 8-week-old female K18 hACE2 transgenic mice (n = 3) were injected (i.p.) with 25 mg/kg of an IgG isotype control, hMAb 1212C2, or hMAb 1213H7 and infected with 10⁴ PFU of rSARS-CoV-2 Venus (top), rSARS-CoV-2 mCherry SA (middle), or both rSARS-CoV-2 Venus and rSARS-CoV-2 mCherry SA (bottom). (A) At 2 and 4 dpi, lungs were collected to determine Venus and mCherry fluorescence expression using an Ami HT imaging system. BF, bright field. (B) Venus and mCherry radiance values were quantified based on the mean values for the regions of interest in mouse lungs. Mean values were normalized to the autofluorescence in mock-infected mice at each time point and were used to calculate fold induction. Gross pathological scores in the lungs of mock-infected and rSARS-CoV-2-infected K18 hACE2 transgenic mice were calculated based on the percentage of area of the lungs affected by infection. Statistical significance was determined by one-way ANOVA analysis on GraphPad Prism. ****, P < 0.0001.