FIG 2.

Transduction of cells by Spike VSV pseudovectors. (A) Indicated cell lines were transduced with equal amounts of SΔ18 VSV-Luc vectors and luciferase activity in cell lysates analyzed 24 h later. Shown are means and standard deviations from 3 independent vector stocks. (B) HeLa and HeLa-ACE2 cells were detached from culture flasks by trypsin, seeded into 96-well plates, and transduced (Td) with equal amounts of SΔ18 VSV-Luc vectors, either immediately (0 h) or 24 h after seeding, and luciferase was measured 24 h later. Data from 9 different wells in a single experiment are shown. ****, P < 0.001, multiple t test. (C) ACE2 expression levels on cell surface measured by flow cytometry. Cells were stained with anti-ACE2 antibody at 0 or 6 h after trypsinization. Mean and standard deviation MFI from four (HeLa) or five (HeLa-ACE2) independent experiments are shown. (D) Lung bud organoids were transduced with equal amounts of VSV-GFP vectors pseudotyped with SΔ18 or control (bald) vectors with no Spike protein. GFP expression was visualized 24 h later. Scale bars represent 100 μm.