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. 2021 Oct 12;11:725336. doi: 10.3389/fonc.2021.725336

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Copyright © 2021 Zhang, Ma, Long, Sun, Liu, Lin and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Runx3 knockdown induces DNA damage and apoptosis in AML cells in vivo. (A) Experimental scheme for getting Runx3 knockdown AML cells induced by MLL-AF9. (B) Representative flow cytometry (FCM) plots and quantitative analysis show the percentage of γ-H2AX⁺ cells in scramble control and Runx3 knockdown AML cells (n = 5 mice). (C) CLSM images of γ-H2AX expression and quantification of γ-H2AX foci per cell (30 cells in each group) in scramble control and Runx3 knockdown AML cells. Nuclear DNA was counterstained with DAPI. Scale bar = 50 μm. (D) Runx3 knockdown increases apoptosis. Representative FCM plots (left) and statistical results (right) show the percentage of apoptotic cells in scramble control and Runx3 knockdown AML cells. Bone marrow cells were stained for Annexin V and 7-AAD (n = 5 mice).