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. 2021 Oct 12;11:725336. doi: 10.3389/fonc.2021.725336

Figure 4.

Figure 4

RUNX3 specifically to DNA repair and anti-apoptosis related genes only in AML cells. Chromatin immunoprecipitation sequencing (ChIP-seq) results for RUNX3 in whole bone marrow cells from normal mice or MLL-AF9 AML mice. (A) Experimental scheme. (B) Number of RUNX3 ChIP-seq peaks and genes identified by HOMER. (C) Pie charts show the genomic distribution of ChIP-seq peaks for RUNX3 in whole bone marrow cells from normal mice (left) or MLL-AF9 AML mice (right). Representation of the annotated regions is shown for comparison. (D) Venn diagram of the RUNX3-bound 5,348 genes in normal bone marrow cells and the RUNX3-bound 10,512 genes in AML bone marrow cells. (E) Gene Ontology (GO) term enrichment analysis of 5,845 genes that can be bound by RUNX3 in leukemia cells but not be bound in normal bone marrow cells. (F) Genome browser views of the distribution of RUNX3 ChIP-seq peaks in DNA repair (Chek1, Ddb1, Rad51c, and Rpa2)- and antiapoptosis (Bcl-2 and Mcl-1)-related gene loci.