Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 23;22(19):10247. doi: 10.3390/ijms221910247

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

NEDD4-1 is highly expressed in temozolomide-resistant patient-derived GBM cell lines. (A) NEDD4-1/PTEN/AKT signaling pathway protein expression survey in glioblastoma cell lines. (B) Viability assay performed using SRB assay showing established TMZ-resistant cell lines, U87MGR and U251R, increased resilience against temozolomide treatment. (C) Tumorsphere formation assay demonstrating the higher potential of TMZ-resistant cell lines to form tumorspheres compared to their chemo-responsive counterpart. (D) Differential expression of NEDD4-1 and PTEN expression in adherent (adh) and tumorsphere (sph) cells in TMZ-responsive and TMZ-resistant U87MG and U251 cell lines. (E) Colony formation assay depicting higher clonogenicity of TMZ-resistant cell lines compared to their TMZ-responsive counterpart. Graph bars are mean ± SEM of 3 independent experiments. *** p < 0.001.