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. 2021 Oct 27;9(2):e00888-21. doi: 10.1128/Spectrum.00888-21

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FIG 3 — Selective permeabilization of the PMN plasma membrane by digitonin. (A) Digitonin and Triton X-100 each permeabilize the plasma membrane of fixed PMNs, as demonstrated by cytoplasmic-actin staining with AF488-phalloidin. Nuclei were stained with NucBlue. (B, C) LAC was labeled with FITC (FITC-LAC) prior to the start of the experiment and is pseudocolored green in all panels. The digitonin treatment conditions used did not permit staining of intraphagosomal bacteria with anti-S. aureus antibody (αSa) coupled to AF594 (B), whereas intraphagosomal bacteria are stained readily after treatment of PMN with Triton X-100 (C). Scale bar = 10 μm.