TABLE 1.

Features of the 1st-generation ADI multi-coronavirus protein microarray^a

Virus	S protein	E protein	M protein	N protein	Accessory or other proteins	No. of features (total n = 934)
SARS-CoV-2	RBDb, Sc from BEI, S1, S2 and 30-, 50-, and 100-aa fragments by IVTT at ADI	Protein and 30- and 50-aa fragments by IVTT at ADI	Protein and 30-, 50-, and 100-aa fragments by IVTT at ADI	Protein and 30-, 50-, and 100-aa fragments by IVTT at ADI	ORF 3a, 6, 7a, 8, and 10 protein and 30-, 50-, and 100-aa fragments by IVTT at ADI	12 proteins, 310 fragments = 322 features
SARS-CoV	Proteind and peptide set from BEI Resources	IVTT by ADI, peptides from BEI Resources	Protein, peptides from BEI Resources	Protein, peptides from BEI	3CL protease from BEI Resources	5 proteins, 265 peptides = 270 features
MERS-CoV	Protein from BEI Resources	Protein produced by IVTT at ADI	Protein produced by IVTT at ADI	BEI Resources	ORF 3a, 4a, 4b, 5, and 8b proteins produced by IVTT at ADI	9 proteins
HCoV-NL63	Protein by IVTT at ADI, peptides from BEI	Protein produced by IVTT at ADI	Protein produced by IVTT at ADI	Protein produced by IVTT at ADI	ORF 3 protein produced by IVTT at ADI	5 proteins and 96 peptides = 101 features
HCoV-OC43	Protein by IVTT at ADI, peptides from BEI	Protein produced by IVTT at ADI	Protein produced by IVTT at ADI	Protein produced by IVTT at ADI	HE, N2 protein IVTT at ADI	6 proteins and 226 peptides = 232 features

^aIVTT means coupled in vitro transcription and translation.

^bRBD is the receptor binding domain, aa 319 to 541 of the SARS-CoV-2 S protein.

^cSARS-CoV-2 S protein is a stabilized form with a trimerization sequence and transmembrane domain deletion.

^dSARS-CoV-S protein is a transmembrane domain deleted form.