TABLE 3.

Predicted change in binding energy relative to Toledo (ΔΔG, in kcal/mol) for interactions between clinical variants of US28 and chemokines or HIV gp120

Variant	CX3CL1 ΔΔG	CCL2 ΔΔG	CCL3 ΔΔG	CCL4 ΔΔG	CCL5 ΔΔG	CCL13 ΔΔG	gp120 Indoa ΔΔG	gp120 Ausb ΔΔG
D15E	−2.8	−4.3	+7.1	−2.6	−1.3	+4.0	+0.5	+1.3
E18L	+7.4	+1.6	+9.7	+0.7	+1.9	−0.8	−4.6	−3.9
D15E, E18Lc	+4.0	−1.4	+15.0	−3.5	−0.7	+3.2	−5.3	−3.7
A19D	−1.2	−4.6	+0.2	+0.2	−6.6	−2.6	−1.0	−1.3
T21A	+2.4	+1.8	+7.5	+2.2	+2.8	+1.9	−0.3	+0.1
F25L	+1.6	+2.4	+1.5	+0.5	+0.4	−2.4	+7.1	+1.2
T21A, F25Lc	+4.1	+4.3	+8.7	+2.3	+3.1	−0.6	+6.5	+1.5
Y40N	+14.5	+9.0	+10.6	+17.4	+9.6	+2.2	+2.9	+9.7
G50C	−0.0	−0.0	−0.0	+8.0	+0.8	−0.0	−0.0	+0.0
N170D	−0.4	+4.5	+2.2	+2.1	−0.1	−4.4	−1.8	−2.4
R267K	+0.9	+0.9	+0.5	+0.6	+0.2	+0.4	−0.3	+0.3

^aIndonesian HIV isolate.

^bAustralian HIV isolate.

^cDouble mutations tested as alleles were universally coinherited.