AF4-D4 results of avidin–polymer conjugates.
| M n a (kg mol−1) | M w a (kg mol−1) | Đ (Mw/Mn) | Polymer chains per avidinb | R g (nm) | R h (nm) | ρ(Rg/Rh)c | Apparent density (kg m−3) | Recoverye (%) | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Zwitterionic polymers | ||||||||||
| C1 | Single-grafted avi-pCBMAshort | 383 | 517 | 1.35 | 7.9 | 16.3 | 15.0 | 0.97 | 22.0 | 93 |
| C2 | Single-grafted avi-pCBMAlong | 741 | 1250 | 1.69 | 6.3 | 23.0 | 24.6 | 0.97 | 18.9 | 92 |
| C3 | Double-grafted avi-pCBMAshort | 159 | 386 | 2.43 | n.d. | 17.2 | 16.5 | 0.94 | 14.0 | 95 |
| C4 | Double-grafted avi-pCBMAlong | 395 | 867 | 2.19 | n.d. | 24.7 | 23.6 | 1.05 | 10.6 | 88 |
| Neutral polymers | ||||||||||
| C5 | Single-grafted avi-pOEGMAshort | 488 | 797 | 1.63 | 6.5 | 19.1 | 20.0 | 0.92 | 21.1 | 90 |
| C6 | Single-grafted avi-pOEGMAlong | 641 | 1540 | 2.40 | 3.5 | 31.2 | 32.5 | 1.07 | 9.3 | 92 |
| C7 | Double-grafted avi-pOEGMAshort | 298 | 904 | 3.03 | 2.3 (4.6d) | 28.9 | 28.0 | 0.91 | 6.6 | 91 |
| C8 | Double-grafted avi-pOEGMAlong | 2320 | 11 800 | 5.09 | 7.8 (15.6d) | 109 | 66.6 | 1.38 | 1.7 | 97 |
a
Calculated with determined dn/dc = 0.154 mL g−1 for pCBMA conjugates and 0.139 mL g−1 for pOEGMA conjugates.
b
Average number of functionalities per avidin molecule (calculated by Mn of conjugate C, subtracted by Mn of macroinitiator (68 kg mol−1) and divided by Mn of specific polymer F).
c
Determined at the peak maximum of DRI signal.
d
Single polymer segments per double-headed initiator.
e
Calculated by dn/dc and exact sample load.