Fig. 8. BUDE_SM PreSaVS for the P2 position. (a) Heat-map for BUDE_SM PreSaVS saturation mutagenesis at P2, showing the ΔΔG = ΔGwt − ΔGmut value calculated for each substitution and each Mpro substrate. Mutations predicted to improve peptide binding have a positive ΔΔG and are greener; those disfavouring binding are in red. (b) The summed ΔΔG values for each residue type substituted at P2.
