Fig. 5.

FTY720 induces protein expression of Vav1 at the site of the wound healing after palatal injury. Representative immunofluorescence images of mice palates 3 days post-injury stained against two important immunomodulators, Vav1 and CD88 proteins. (a–d) and (i–l) panels are from injured palate treated with blank nanofiber scaffold (vehicle). (e–h) and (m–p) panels are from injured palate treated with FTY720 nanofiber scaffold. The panels (a, e, i, and m) are nuclear staining using DAPI (blue). Panels (b, f, g, and n) are staining against CD88-Alexa488 (green). Panels (c, g, k, and o) are staining against Vav1-Alexa568 (red). Panels (d, h, l, and p) are the merged images. The asterisk symbol signalizes the same region in the injured palate treated with FTY720 between ×10 (H) and ×100 (P) panels. The images were acquired using the ×10 and ×100 objective on an Olympus F1000 microscope. The scale bars are noted in yellow, 10 μm for ×10 (A) and 100 μm for ×100 (M) images