Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 16;156(4):333–347. doi: 10.1007/s00418-021-02015-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — EBF1-expressing cells are pericytes. Double immunostaining combining the specific antibody for EBF1 and lineage-specific markers was applied for glioblastoma samples with prominent glomeruloid vascular proliferation in order to identify the phenotype of EBF1-expressing cells. Double immunostaining using endothelial markers CD31, CD34, FVIII showed no double-positive cells (a), and the same was found for monocyte/macrophage markers (CD68, CD163, Tie-2) (b) and for the histological hallmarks of tumor-derived glioma cells (GFAP, EGFRvIII, IDH1-R132H) (c). In contrast, double immunostaining using the most widely recognized mesenchymal/pericyte markers (PDGFRβ, SMA, CD146, NG2 and CD90) (d) revealed the pericyte phenotype of EBF1-expressing cells. Chromogen used to identify immunoreactivity is either brown or blue according to the corresponding label, as indicated. All images are ×40 original magnification; scale bar corresponds to 50 μm