. 2021 Sep 8;6(5):e00654-21. doi: 10.1128/mSphere.00654-21

TABLE 3.

Transcriptional regulation of fdrA-lacZ reporter gene fusion in pMW151 contained in E. coli MC4100

Effector (20 mM)	β-Galactosidase activity (MU)^a
Allantoin	9,511.1 ± 1,062.6
Oxamate	71.3 ± 19.9
NH₄Cl	72.8 ± 37.3
Oxalate^b	85.4 ± 54.1

The bacteria were grown on glycerol (50 mM) and DMSO (50 mM) in M9 medium to an OD₆₀₀ of 0.4 to 0.6 under anaerobic conditions. Cultures contained 20 mM allantoin, oxamate, NH₄Cl, or oxalate as the effector. The β-galactosidase activity of the empty lacZ fusion plasmid pJL29 was measured for each culture condition and as a blank value. Values are averages and SD, derived from at least three independent cultures, each measured in quadruplicate. MU, Miller units.

eM9 (enriched M9 medium) was used for cultivation.