TABLE 4.
Effect of fdrA variation on anaerobic allantoin degradation
| Strain | Allantoin consumed (mM) | Production (mM) |
Glycerol consumed (mM) | Production (mM) |
Growth | pH | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Oxamate | Oxalate | Succinate | Formate | Acetate | Ethanol | |||||
| MG1655 (ancestor) | 21.1 ± 1.0a | 16.6 ± 1.2 | 3.2 ± 1.4 | 29.4 ± 1.2 | 6.2 ± 0.7 | 26.8 ± 0.4 | 15.3 ± 1.0 | 12.6 ± 0.6 | 0.7 ± 0 | 6.2 ± 0.1 |
| MG1655 ΔfdrA | 21.0 ± 0.9 | 0 | 10.9 ± 0.8 | 28.6 ± 1.2 | 5.4 ± 0.8 | 27.2 ± 1.8 | 14.4 ± 0.6 | 13.7 ± 0.6 | 0.6 ± 0 | 6.1 ± 0.1 |
| MG1655 ΔfdrA pNTR-SD::fdrA | 20.2 ± 0.2 | 5.6 ± 1.3 | 7.9 ± 0.2 | 28.5 ± 0.7 | 6.3 ± 0.1 | 20.3 ± 0.6 | 7.5 ± 0.2 | 12.0 ± 0.1 | 0.6 ± 0 | 6.0 ± 0 |
| MG1655 fdrAD296Y | 20.8 ± 0.8 | 20.9 ± 1.7 | 0.4 ± 0.8 | 36.3 ± 2.0 | 10.0 ± 0.3 | 30.0 ± 1.6 | 20.3 ± 0.4 | 11.9 ± 0.6 | 0.6 ± 0 | 5.9 ± 0 |
a
Bacterial strains (wild type, FdrAD296Y, ΔfdrA, and ΔfdrA containing pNTR-SD::fdrA) were anaerobically grown in M9 containing glycerol (50 mM), DMSO (50 mM), and allantoin (20 mM) for 72 h, whereby allantoin was used as a sole nitrogen source. Values are averages and SD for 3 replicates.