TABLE 3.
Half-lives and relative steady-state amounts of mRNA and pre-mRNA and steady-state amounts of iso-1-cytochrome c in cyc1-512 strains having single and double cbc1-Δ and upf1-Δ mutations
| mRNA | Size (nt) | Half-life (min)
|
Relative steady-state amt
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| B-9037 (CBC1+ UPF1+) | B-9038 (cbc1-Δ UPF1+) | B-9848 (CBC1+ upf1-Δ) | B-11559 (cbc1-Δ upf1-Δ) | B-9037 (CBC1+ UPF1+) | B-9038 (cbc1-Δ UPF1+) | B-9848 (CBC1+ upf1-Δ) | B-11559 (cbc1-Δ upf1-Δ) | ||
| cyc1-512 mRNAs | |||||||||
| 1 | 630a | 33.1 ± 0.03 | 35.6 ± 0.6 | 29.6 ± 1.08 | 35.5 ± 0.72 | 45 | 158 | 40 | 125 |
| 630b | 33.8 ± 0.09 | 36.1 ± 0.03 | 21.5 ± 0.9 | 16.7 ± 0.3 | 43.4 | 128 | 37 | 118 | |
| 2 | 850b | 9.6 ± 0.1 | 8.88 ± 0.06 | 8.8 ± 0.3 | 6.9 ± 0.5 | 19.3 | 73 | 26 | 100 |
| 3 | 1,450b | 4.4 ± 0.003 | 12.8 ± 0.7 | 13.8 ± 1.5 | 12.4 ± 1.3 | 5.5 | 14.0 | 5.6 | 15.8 |
| 4 | 1,650b | 6.1 ± 0.05 | 17.4 ± 0.05 | 16.2 ± 2.0 | 12.1 ± 0.5 | 5.4 | 13 | 11.3 | 14.1 |
| 5 | 1,850b | 6.6 ± 0.05 | 20.2 ± 0.2 | 17.3 ± 0.9 | 14.2 ± 1.0 | 6.5 | 12 | 16.6 | 20 |
| 6 | 2,000b | ||||||||
| 7 | 2,400b | 6.07 ± 0.09 | 12.3 ± 1.5 | 18.4 ± 1.6 | 22.5 ± 1.3 | 7.7 | 20 | 19 | 26 |
| 8 | 3,500b | 5.5 ± 0.2 | 18.7 ± 1.4 | 12.5 ± 0.1 | 15.9 ± 3.6 | 5.9 | 21.6 | 24.5 | 21 |
| CYH2 mRNAa | 36.5 ± 1.3 | 46 ± 2.0 | 42 ± 3.06 | 41.5 ± 2.2 | 9.2 | 7.9 | 9.4 | 8.56 | |
| CYH2 pre-mRNAa | 3.0 ± 0.006 | 4.3 ± 0.007 | 12.7 ± 0.02 | 14.0 ± 0.03 | 3.0 | 2.9 | 76.0 | 73.2 | |
| ACT1 mRNAa | 41 ± 1.6 | 55 ± 1.4 | 33 ± 0.3 | 57 ± 1.6 | 7.9 | 6.8 | 7.2 | 7.1 | |
| Iso-1-cytochrome c | 10% | 45% | 30% | 45% | |||||
a
Determined from total RNA.
b
Determined from mRNA.