FIGURE 6:
Example results and data for the study of astrocytes by the FACT tissue clearing method. (A) A cleared tissue of 2 mm thickness. (B)-(C) 3-dimensional image of astrocytes labeled with aldh1l1-tdTomat (knock-in) and anti-GFAP Alexa 488. (D) A 2D image of astrocytes labeled with anti-GFAP Alexa 488 showing astrocytic processes. (E)-(F) Example data for morphology characterization by Imaris.