Table 3:
Excitation and emission wavelengths of common fluorophores
| Fluorophore | Absorption wavelength (nm) |
Emission wavelength (nm) |
Visible color |
|---|---|---|---|
| Hydroxycoumarin | 325 | 386 | Blue |
| Methoxy coumarin | 360 | 410 | Blue |
| Alexa fluor | 345 | 442 | Blue |
| DAPI | 345 | 455 | Blue |
| Hoechst 33258 | 345 | 478 | Blue |
| Aminocoumarin | 350 | 455 | Blue |
| SYTOX blue | 431 | 480 | Blue |
| Hoechst 33342 | 343 | 483 | Blue |
| YOYO-1 | 489 | 509 | Dark green |
| Cynin 2 | 490 | 510 | Dark green |
| FAM | 495 | 516 | Dark green |
| Alexa fluor 488 | 494 | 517 | Light green |
| Fluorescein FITC | 495 | 518 | Light green |
| SYTOX green | 504 | 533 | Light green |
| TOTO 1, TO-PRO-1 | 509 | 533 | Light green |
| Alexa fluor 430 | 430 | 545 | Light green |
| Alexa fluor 532 | 530 | 555 | Light green |
| HEX | 535 | 556 | Light green |
| Cynin 3 | 550 | 570 | Yellow |
| SYTOX Orange | 547 | 570 | Yellow |
| TRITC | 547 | 572 | Yellow |
| Alexa fluor 546 | 556 | 573 | Yellow |
| Alexa fluor 555 | 555 | 565 | |
| Chromomycin A3 | 445 | 575 | Yellow |
| Mithramycin | 445 | 575 | Yellow |
| R-phycoerythrin (PE) | 480,565 | 578 | Yellow |
| Rhodamine Red-X | 560 | 580 | Orange/Red |
| Tamara | 565 | 580 | Orange/Red |
| Cynin 3.5 | 581 | 596 | Red |
| Rox | 575 | 602 | Red |
| Alexa fluor 568 | 578 | 603 | Red |
| Red 613 | 480,565 | 613 | Red |
| Texas Red | 589 | 615 | Red |
| Alexa fluor 594 | 590 | 617 | Red |
| Alexa fluor 633 | 621 | 639 | Red |
| Cynin 5 | 650 | 670 | Red |
| Alexa fluor 660 | 663 | 690 | Red |
| Cynin 5.5 | 675 | 694 | Red |
| Propidium iodide | 536 | 617 | Red |
| Ethidium bromide | 493 | 620 | Red |
| Alexa fluor 680 | 679 | 702 | Red |
| Cynin 7 | 743 | 770 | Red |