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. 2021 Oct 13;9:739357. doi: 10.3389/fcell.2021.739357

FIGURE 1.

FIGURE 1

Recovery of Drosophila blood cell by bleeding. (A–D) Confocal images of cells recovered from srpD-GAL4,UAS-mCherry (A, srpD-GAL4) or srpHemo-His2A-RFP (B–D, srpHemo) adult females. Cells were counterstained with phalloidin (green) and DAPI (blue). The age of the flies is indicated below each panel. Scale bar: 200 μm. The arrow in panel (B) indicates a fat body cell surrounded by hemocytes. (E) Quantifications of the proportion of cells (DAPI+) expressing srpHemo-His2A-RFP according to fly age. Means and standard deviations of five independent experiments are shown. A total of 2316, 2351, and 1516 cells were counted in 2- day-, 5- day-, and 35-day-old flies, respectively. (F) Quantifications of the absolute number of cells recovered per fly at different ages. Means and standard deviations from eight independent experiments are represented. Kruskal–Wallis test was used for statistical analysis. n.s., non-significant (p > 0.05); **p < 0.01.