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. 2021 Oct 13;9:739357. doi: 10.3389/fcell.2021.739357

FIGURE 6.

FIGURE 6

Fusiform cells are derived from the lymph gland posterior signaling center. (A–F,L–W) Cell lineage analyses of adult blood cells origin. Confocal views showing the different types of fusiform (A,D,G,J–L,O,R,U) or round (B,C,E,F,H,I,M,N,P,Q,S,T,V,W) cells recovered in adult flies expressing the G-TRACE reporter transgenes under the control of the indicated drivers. Present and past expression of the drivers are revealed by nuclear RFP (red, A’–F’ and L’–W’) and nuclear GFP (green, A”–F” and L”–W”), respectively. (G–H) Expression of Col (green) in adult blood cells as revealed by immunostaining. (I–K) Confocal views of adult bleeds (I,J) and abdominal hematopoietic hub (K) from Pxn-RedStinger, pcol85-GAL4,UAS-mCD8-GFP flies. (A–W) Scale bar 20 μm. Cells were counterstained with phalloidin (A–H,L–W: red; I–K: white) and DAPI (blue).