TABLE 2.
Summary of costs and results for different collection methods
| Method | Per-unit cost ($)a |
Resultd |
|||||
|---|---|---|---|---|---|---|---|
| Metagenomics |
Metabolomics |
||||||
| Stability | Concordance | Reliability | Stability | Concordance | Reliability | ||
| 95% ethanol | 0.23b | − | ++ | − | ++ | ++ | + |
| RNAlater | 5.40b | ++ | ++ | + | + | + | − |
| Microlution | 12.14c | + | ++ | + | + | + | + |
| OMNIgene | 20.00 | ++ | ++ | ++ | + | + | − |
| FOBT card | 7.52c | ++ | + | + | NA | NA | NA |
Costs for all methods were derived from Fisher Scientific, except for Microlution and OMNIgene Gut. The cost estimate for Microlution was sourced from https://www.biomart.cn/infosupply/48662146.htm. The cost estimate for OMNIgene Gut was sourced from peer-reviewed research (62).
Two-milliliter volume; did not include containment and collection devices.
Did not include collection device.
++ (good) means that the number of ICCs of ≥0.75 is ≥10 in the abundance of the top 4 phyla and 15 microbial diversity metrics at the species, gene, and pathway levels or that the median ICC across metabolites is >0.70. + (medium) means that the number of ICCs of ≥0.75 is 6 to 11 in the abundance of the top 4 phyla and 15 microbial diversity metrics at the species, gene, and pathway levels or that the median ICC across metabolites is 0.45 to 0.70. − (poor) means that the number of ICCs of ≥0.75 is <6 in the abundance of the top 4 phyla and 15 microbial diversity metrics at the species, gene, and pathway levels or that the median ICC across metabolites is <0.45. NA, not applicable.