FIG 1.

Ribosome profiling data set. (A) A fluorescence-activated cell sorting analysis of DNA content staining with propidium iodide was carried out for HU-synchronized T. cruzi populations. The distributions of G₁ (2C cells, white), S (2-4C cells, gray), and G₂/M (4C cells, black) are presented for an asynchronous parasite culture (Asyn), a G₁-enriched population (0 h post-HU), and an S-phase-enriched population (6 h post-HU). (B) Principal-component analysis of the gene expression values for the replicates of G₁-enriched population (gray squares) and S-enriched population (black diamonds). (C) Volcano plot showing the distribution of FDR values for each gene versus the fold change in expression. Red dots are genes classified as DEGs (FC > 2, FDR < 0.05, nRFPs > 40). (D) Heatmap representing the 50 up- and downregulated genes (log₂ nRFP values are presented). (E) Observed (obs)-to-expected (exp) ratio of the 5′-end footprint mapping distribution in the three reading frames, analyzed for the ribosome profiling (R) and the transcriptome (T). (F) Average mapping position was taken and counted among coding (CDS, dark gray) and noncoding (non-CDS, light gray) sequences for the ribosome profiling (R) and the transcriptome (T).