FIG 5.
UV inactivation of producer virus and activation of DI with activator virus. (A) Effects of various UV dosages on rCDVRI producer virus titers. Samples were irradiated using a UV crosslinker (CX-2000 crosslinker; UVP), and TCID50s per milliliter were determined. The dotted line represents the limit of detection of the assay. Error bars represent standard deviations (n = 3). (B) UV doses were tested in the presence of a DI genome (red) empirically to determine the required amount to inactivate the rCDVRI producer virus and not the DI genome. Vero-cCD150 cells infected with UV-treated samples were superinfected with the rCDVRITagBFP activator virus, in order to drive the replication of the DI genome in the samples. (C) The stability of the DI genome in infected cells was tested. DI genomes remained dormant in cells for up to 3 days, as shown by genome activation with rCDVRI. (D) Activation of the rCDVRI DI genome using various paramyxoviruses as the activator virus. We tested the cross-reactivity of rCDVRI DIP to other paramyxoviruses: measles virus (MV), mumps virus (MuV), and human respiratory syncytial virus (HRSV). Activation of an rCDVRI DI genome occurred only with CDV and MV, both morbilliviruses.