FIG 7.

rCDV^RI infection in ferrets. (A) Experimental outline of ferret infection. Ferrets were either pre- or coadministered with DIP nDI^RI04cb and preadministered with sDI^RIdTomdel04cb or culture medium prior to infection with the challenge virus rCDV^RITagBFP. Preadministration was carried out 6 h before infection. (B) Weight change of ferrets during the course of infection. (C) Lymphocyte percentage in EDTA-blood determined by flow cytometry show lymphopenia. (D) Virus infection in lymphocytes was determined for each time point by monitoring fluorescence using flow cytometry. A peak in infection is seen at day 6. (E) sDI^RIdTom04cb and virus were isolated from WBC (6 dpi) and from lymph nodes (14 dpi) from ferrets. TB-LN, tracheal-bronchial lymph node; RP-LN, retropharyngeal lymph node. (F and G) sDI^RIdTom04cb genome copies were detected in WBC at each time point during the course of the infection and at necropsy in single-cell suspensions of lymphoid tissues. (H and I) nDI^RI04cb genome copies were detected in WBC at each time point during the course of the infection and at necropsy in lymphoid tissues.