TABLE 2.

Defective rule-of-six-complaint CDV trailer copyback genomes

Genome	Genome length (nt)	Breakpoint (nt position)	Reinitiation (nt position)	Overlapa	Stem length (nt)b	Loop length (nt)b	Detection methodc	Named
1	324	15454	15602	2	89	148	NGS
2	480	15306	15594	2	97	288	NGS
3	486	15441	15451	4	240	10	NGS
4	504e	15332	15546	0	145	124	RT-PCR
5	528e	15331	15523	0	168	192	RT-PCR
6	630	15255	15495	2	196	240	RT-PCR, NGS	nDIRI04cb
7	690	15110	15582	0	109	472	RT-PCR, NGS	nDIRI11cb
8	720	15295	15366	1	325	71	RT-PCR, NGS	nDIRI07cb
9	786	15289	15306	1	385	17	NGS
10	792	15008	15582	0	109	574	NGS
11	816e	14969	15597	0	94	628	RT-PCR
12	906e	14913	15563	0	128	650	RT-PCR
13	936e	14933	15454	0	237	461	RT-PCR
14	1,092	14825	15465	0	226	640	RT-PCR, NGS	nDIRI10cb
15	1,134	15123	15125	0	566	2	NGS
16	1,254	14706	15419	3	272	713	NGS
17	1,422	14823	15136	0	555	313	NGS
18	1,554e	14733	15095	0	596	362	RT-PCR
19	1,734	14166	15482	0	209	1,316	NGS
20	2,124f	14620	14620	0	1,071	0	NGS
21	2,298	14266	14817	0	874	551	NGS

^aOverlap, the number of nucleotides at the breakpoint that may come from either the positive- or the negative-sense strand.

^bThis number is ± the overlap.

^cNGS, next-generation sequencing.

^dNamed in the order in which they were identified.

^ecbDI genome identified from an rCDV^RIVenus(6) lab stock.

^fSnap-back genome.