FIG 3.

Subclass-switched MAbs display similar binding and neutralization but increased ability to activate effector functions. (A) Subclass-switched MAbs had similar neutralizing ability as assessed by FRNT. Mean and SD in triplicate are reported. (B) Subclass-switched MAbs had similar Gn binding as tested by ELISA of RVFV-infected lysates. Geometric means are shown with a horizontal line, and error bars represent the geometric SD for each MAb. The horizontal dashed line represents the LOD of this assay. (C) Assessment of MAb ADCC by NK cell degranulation assay. Each point represents an independent replicate, with means shown by a horizontal line and SD by the error bars. The gray horizontal line indicates the range of degranulation induced by IgG1 and IgG2a isotype control Abs. Statistical significance in the ability of MAbs to induce degranulation in NK cells was assessed by unpaired Student’s t tests. (MAb-1, P = 0.0077; MAb-2, P = 0.0012; MAb-3, P = 0.0286).