Skip to main content
. 2021 Oct 27;7(44):eabh0050. doi: 10.1126/sciadv.abh0050

Fig. 3. Cherry-expressing cells originate from the hemolymph.

Fig. 3.

(A and B) Schematic view on the experimental strategies. The light orange–colored structure corresponds to the inflamed brain. The blue-colored brain reflects a control animal without infection. (A) Injection of fluorescent latex beads into the hemolymph of wandering third instar larvae. (B) Transplantation of macrophages expressing the srpHemo-moe::3xmCherry into the hemolymph of wandering third instar larvae. (C to F) Twenty-four hours APF pupal brains were stained for horseradish peroxidase (HRP) (cyan) and mCherry (magenta). (C) Control brain with no immunity induction. (D and E) Following pan-glial immunity induction, macrophages containing 1-μm green latex beads (D and D′) (arrows indicate macrophages containing latex beads) or 0.5-μm orange latex beads (E) (see arrows) are found in the CNS. (F and F″) Pupal brain after immunity induction and transplantation of genetically labeled macrophages (arrows). Several labeled macrophages are found in the ventral nerve cord. The position of the orthogonal sections shown in (F’) and (F″) is indicated by a white dashed line. Scale bars, 100 μm.