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. 2021 Oct 27;7(44):eabh0050. doi: 10.1126/sciadv.abh0050

Fig. 5. Relish is required for macrophage invasion.

Fig. 5.

(A, B, D, and E) Twenty-two to 24-hour-old pupal brains stained as indicated. Glial nuclei (anti-Repo, green), neuronal membranes (anti-HRP, cyan), and invading macrophages (mCherry or dsRed, magenta). Scale bar, 100 μm. (A) Upon concomitant expression of PGRP-LE and silencing of relish expression using RNA interference, no macrophages enter the brain. (B and C) No suppression of macrophage invasion induced by PGRP-LE expression is observed following concomitant suppression of basket expression. For quantification, see (C) (n = 6; P = 0.6753, Mann-Whitney). ns, not significant. (D) Pan-glial expression of activated Relish causes an invasion of macrophages into the brain. (E) Neuronal expression of activated Relish is not sufficient to trigger invasion of macrophages into the CNS.