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. 2021 Oct 26;32(11):134. doi: 10.1007/s10856-021-06611-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The evaluation of VEGFR1 protein expression in healthy and MI Cell lysates from 2D and 3D cultures (A) and VEGF protein conditioned media from 2D and 3D cultures (B). The VEGFR1 protein expression from MI and healthy cell lysates cultured in the 3D system was significantly higher than VEGF protein expression in MI and healthy cell lysates cultured in the 2D system. The expression levels of VEGF protein were severely elevated in conditioned media from the 3D cultures. Control/Healthy cell: cardiomyocytes isolated from healthy NMRI mice. MI cell: cardiomyocytes isolated from myocardial infarction NMRI mice. The values are shown as the means and standard deviation of the mean (mean ± SD). *p < 0.05, **p ≤ 0.01, and ***p ≤ 0.0001 shows the significant change in comparison to control (p < 0.05). ^#shows the significant change in comparison to the MI group (p < 0.05)