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. 2020 Jul 11;15(9):1792187. doi: 10.1080/15592324.2020.1792187

Figure 4.

Figure 4.

Exogenous application of 12-OPDA, 9,12-KOMA, and 9,12-KODA increases maize resistance to C. graminicola. (a) Lesions caused by C. graminicola infection of leaves of untreated by T. virens W438 inbred line and lox10-3 mutants in W438 background sprayed with 1 mL of 20 µM 12-OPDA (dissolved in ethanol) or ethanol (control). Spores of C. graminicola (approximately 1 × 106 spores/mL) were drop inoculated on leaves 6 h after spray treatment, and leaves were scanned 5 d after infection. (b) Bar graph represents average lesion area of W438 inbred maize and lox10-3 mutants in the W438 background sprayed with 20 µM 12-OPDA (dissolved in ethanol) or ethanol (control) and infected with C. graminicola. Error bars represent standard error. Letters indicate significant differences among all treatments (Tukey’s HSD test, p < .05). (c) Bar graph represents average lesion area of B73 plants transfused with sap supplemented with 100 nM or 10 nM 9,12-KODA or 9,12-KOMA and infected by C. graminicola, with error bars representing standard error. Letters indicate significant differences among all treatments (Tukey’s HSD test, p < .05).